Concept

sample preparation method entity

URI	http://vocab.nerc.ac.uk/collection/S01/current/S019/
Within Vocab	BODC parameter semantic model component names
Alternative Labels
Definition	Portion of the BODC parameter semantic model describing pre-analysis processing. It is part of the how theme.
Date	2018-08-23T15:25:14
Identifier	SDN:S01::S019
Note	accepted
Has Current Version	2
Version	1
version	2
Broader	`S01:S013`	how theme
Narrower	`S03:S03116`	filtration, oxidation
	`S03:S0393`	filtration
	`S03:S0383`	diffusive gel thin-film (DGT) sampling, extraction into HNO3
	`S03:S0382`	compression into pellets
	`S03:S0398`	filtration, acetone extraction
	`S03:S0372`	centrifugation, acetone extraction
	`S03:S03100`	filtration, acidification
	`S03:S03103`	filtration, acidification, chelation, solvent extraction
	`S03:S03221`	wet sieving, acid digestion
	`S03:S0360`	acid digestion
	`S03:S0362`	acid leaching of filter residue
	`S03:S03113`	filtration, methanol extraction
	`S03:S03440`	filtration, indophenol synthesis, SPE extraction, HPLC purification
	`S03:S03121`	filtration, thin-film preparation
	`S03:S03446`	filtration, UV digestion
	`S03:S0366`	acidification
	`S03:S03220`	wet sieving
	`S03:S03435`	acidification, isotope dilution
	`S03:S0359`	UV digestion
	`S03:S03132`	in-situ pore water sampling, filtration, chelation, solvent extraction
	`S03:S0330`	sediment trapping
	`S03:S03106`	filtration, in-line preconcentration
	`S03:S0371`	centrifugation
	`S03:S03429`	filtration, acidification, UV irradiation
	`S03:S0368`	centrifugal extraction
	`S03:S03442`	filtration, ethanol extraction
	`S03:S03420`	filtration, sudan-1 synthesis, SPE extraction, HPLC purification
	`S03:S0388`	equilibration with air, drying
	`S03:S03122`	flux-diluted fused bead preparation
	`S03:S03149`	oxalic acid extraction
	`S03:S0392`	extraction, filtration
	`S03:S03150`	oxidation
	`S03:S0369`	centrifugal extraction, filtration
	`S03:S03171`	shower-head equilibration with air, drying
	`S03:S03418`	solvent extraction
	`S03:S03438`	filtration, N, N-dimethylformamide extraction
	`S03:S0389`	extraction by squeezing, filtration
	`S03:S03222`	wet sieving, acid digestion, stannous chloride reduction
	`S03:S0370`	centrifugal extraction, filtration, acidification
	`S03:S03225`	wet sieving, ignition at 400C
	`S03:S0331`	sieving and picking under an optical microscope
	`S03:S03115`	filtration, ortho-phthalaldehyde reaction
	`S03:S039`	identification by optical microscopy
	`S03:S03413`	filtration, water leaching
	`S03:S03131`	in-situ pore water sampling, filtration
	`S03:S03448`	filtration, acid leaching
	`S03:S0341`	leaching with 1M ammonium acetate at pH 7
	`S03:S03185`	isotope-labelled tracer addition, in-situ incubation, filtration
	`S03:S03411`	Soxhlet extraction
	`S03:S03235`	parallel screened water incubations with and without added grazers, grazer removal, filtration, acidification
	`S03:S0399`	filtration, acid digestion
	`S03:S03195`	tracer doping, incubation at 80uE/m2/s light level, cascade filtration
	`S03:S03118`	filtration, purging, cryogenic trapping
	`S03:S03178`	tracer doping, antibiotic poisoning, incubation at 188uE/m2/s light level, filtration
	`S03:S03184`	isotope-labelled tracer addition, in-situ incubation, cascade filtration
	`S03:S03417`	filtration, leaching with pH 4.7 ammonium acetate buffer
	`S03:S03412`	cascade impaction
	`S03:S0333`	unspecified staining
	`S03:S03224`	wet sieving, drying
	`S03:S03210`	isotope-labelled tracer addition, incubation in the dark, filtration
	`S03:S03111`	filtration, incubation in the dark, purging, cryogenic trapping
	`S03:S033`	acetone extraction
	`S03:S0332`	size fractionation
	`S03:S03134`	incubation at full natural light level
	`S03:S0390`	extraction by squeezing, filtration, acidification
	`S03:S03123`	hydrolysis
	`S03:S0403`	centrifugal extraction, filtration, oxidation
	`S03:S03236`	parallel screened water incubations with and without added grazers, grazer removal, filtration
	`S03:S0319`	on-deck incubation without nutrients
	`S03:S03419`	filtration, incubation at unspecified light level, purging, cryogenic trapping
	`S03:S03194`	tracer doping, incubation at 530uE/m2/s light level, filtration
	`S03:S03188`	tracer doping, incubation at 100uE/m2/s light level, filtration
	`S03:S03156`	purging, cryogenic trapping
	`S03:S03198`	isotope-labelled tracer addition, incubation at different light levels, filtration
	`S03:S0310`	incubation
	`S03:S03202`	isotope-labelled tracer addition, incubation at unspecified light level, filtration
	`S03:S03137`	light and dark bottle incubation
	`S03:S03114`	filtration, no acidification
	`S03:S0397`	filtration, Mn absorption
	`S03:S0363`	acid liberation, purification and trapping CO2
	`S03:S03204`	tracer doping, incubation at unspecified light level, filtration, acidification
	`S03:S0386`	drying
	`S03:S03197`	isotope-labelled tracer addition, incubation at different light levels, cascade filtration
	`S03:S03139`	no acidification
	`S03:S03216`	isotope-labelled tracer addition, on-deck incubation at simulated in-situ light level, filtration
	`S03:S0373`	centrifugation, acid digestion
	`S03:S0334`	vacuum filtration
	`S03:S0377`	centrifugation, no acidification
	`S03:S03406`	SYBR Green I staining
	`S03:S03200`	isotope-labelled tracer addition, incubation at unspecified light level, cascade filtration
	`S03:S03182`	isotope-labelled tracer addition, azide poisoning, incubation at unspecified light level, cascade filtration
	`S03:S0321`	picking
	`S03:S0339`	14CO3 doping, incubation at 100uE/m2/s, acidification, scintillant addition
	`S03:S03125`	ignition at 400C
	`S03:S03189`	tracer doping, incubation at 188uE/m2/s light level, cascade filtration
	`S03:S038`	grazing experiment based on the method of Gifford (1993)
	`S03:S03177`	tracer doping, antibiotic poisoning, incubation at 188uE/m2/s light level, cascade filtration
	`S03:S03219`	3H leucine doping, incubation in the dark, centrifugation
	`S03:S032`	DAPI/proflavine staining
	`S03:S0394`	filtration with acid impregnated filter
	`S03:S03135`	incubation under ultra-violet light
	`S03:S03212`	tracer doping, on-deck incubation at full natural light level, cascade filtration
	`S03:S0361`	acid digestion of filter residue
	`S03:S0395`	filtration with Teflon filter
	`S03:S03227`	melting of core section
	`S03:S0311`	incubation at constant temperature
	`S03:S03432`	filtration, DNA extraction
	`S03:S03186`	tracer doping, incubation at 100uE/m2/s light level, cascade filtration
	`S03:S03447`	acid digestion of leached filter residue
	`S03:S0380`	combustion
	`S03:S03152`	digestion, purification, plating
	`S03:S03191`	tracer doping, incubation at 188uE/m2/s light level, filtration
	`S03:S03213`	tracer doping, on-deck incubation at full natural light level, filtration
	`S03:S03456`	marine snow catcher sampling, settling
	`S03:S03183`	isotope-labelled tracer addition, azide poisoning, incubation at unspecified light level, filtration
	`S03:S03231`	eosin staining
	`S03:S03217`	isotope-labelled tracer addition, on-deck incubation at simulated in-situ light level, filtration, acidification
	`S03:S03428`	purging, sorbent tube trapping
	`S03:S03167`	sediment trapping, oxidation
	`S03:S03154`	polyacrylamide gel probe sampling
	`S03:S03215`	isotope-labelled tracer addition, on-deck incubation at simulated in-situ light level, cascade filtration
	`S03:S03166`	sediment trapping, no acidification
	`S03:S03133`	incubation in the dark
	`S03:S03409`	filtration, DNA extraction, PicoGreen staining
	`S03:S03426`	filtration, light and dark bottle incubation
	`S03:S03175`	tracer doping, antibiotic poisoning, incubation at 100uE/m2/s light level, cascade filtration
	`S03:S03153`	plating
	`S03:S034`	acridine orange staining
	`S03:S03449`	filtration, denitrification to N2O by cultured bacteria
	`S03:S03436`	filtration, acidification, isotope dilution
	`S03:S03430`	denitrification to N2O by cultured bacteria
	`S03:S03408`	laboratory rock sample leaching
	`S03:S0326`	preservation and no staining
	`S03:S031`	DAPI staining
	`S03:S03404`	high temperature reduction in contact with zinc
	`S03:S0329`	screening, on-deck incubation dilution experiment
	`S03:S03444`	isotope-labelled tracer addition, incubation, filtration, acidification
	`S03:S0347`	AgI precipitation
	`S03:S03410`	filtration, DNA extraction, PicoGreen staining, primer selection
	`S03:S03193`	tracer doping, incubation at 530uE/m2/s light level, cascade filtration
	`S03:S03452`	acidification, NaOH hydrolysis
	`S03:S03425`	filtration, Bligh and Dyer extraction
	`S03:S03434`	filtration, acidification, pre-concentration, ion-exchange chromatography
	`S03:S03401`	isotope-labelled tracer addition, on-deck incubation at simulated in-situ light level, cascade filtration, acidification
	`S03:S03441`	isotope-labelled tracer addition, incubation, filtration, indophenol synthesis, SPE extraction
	`S03:S03228`	micromilling
	`S03:S03450`	filtration, NaOH hydrolysis, NaBH4 reduction
	`S03:S03433`	anion exchange chromatography
	`S03:S03431`	sediment trapping, digestion, purification, plating
	`S03:S03208`	tracer doping, incubation in the dark, cascade filtration
	`S03:S0314`	no preservation and no staining
	`S03:S03107`	filtration, incubation
	`S03:S03102`	filtration, alkali hydrolysis
	`S03:S03192`	tracer doping, incubation at 23uE/m2/s light level, filtration
	`S03:S0352`	Millipore prefiltration
	`S03:S03110`	filtration, incubation in the dark, enzyme reduction to DMS
	`S03:S03105`	filtration, freon extraction
	`S03:S03161`	sediment trapping, acid digestion
	`S03:S03174`	isotope-labelled tracer addition, DCMU poisoning, incubation at unspecified light level, filtration
	`S03:S03181`	tracer doping, azide poisoning, incubation at 100uE/m2/s light level, cascade filtration
	`S03:S03453`	epibenthic sampling
	`S03:S03129`	in-situ light and dark bottle incubation
	`S03:S03173`	isotope-labelled tracer addition, DCMU poisoning, incubation at unspecified light level, cascade filtration
	`S03:S0349`	EDTA/SDS extraction
	`S03:S0391`	extraction by squeezing, filtration, barium/gelatine treatment
	`S03:S03445`	15N2-enriched seawater addition, on-deck incubation at simulated in-situ light level, filtration
	`S03:S03405`	equilibration with and cryogenic trapping of CO2
	`S03:S03119`	filtration, reaction with orthophthalic acid
	`S03:S03454`	filtration, precipitation, acidification
	`S03:S0374`	centrifugation, acidification
	`S03:S03226`	wet sieving, no acidification
	`S03:S03407`	high temperature reduction in contact with carbon
	`S03:S0376`	centrifugation, combustion
	`S03:S0365`	acidification, combustion
	`S03:S0315`	no staining
	`S03:S03164`	sediment trapping, combustion
	`S03:S0384`	disaggregation
	`S03:S03117`	filtration, plating
	`S03:S03172`	siphoning
	`S03:S03179`	isotope-labelled tracer addition, antibiotic poisoning, incubation in the dark, cascade filtration
	`S03:S0375`	centrifugation, acidification, combustion
	`S03:S03104`	filtration, combustion
	`S03:S03176`	tracer doping, antibiotic poisoning, incubation at 100uE/m2/s light level, filtration
	`S03:S03218`	volume measurement, drying
	`S03:S03455`	sieving, picking
	`S03:S0328`	screening, in-situ incubation dilution experiment
	`S03:S03196`	tracer doping, incubation at 80uE/m2/s light level, filtration
	`S03:S0381`	combustion at 375C and acidification
	`S03:S03108`	filtration, incubation in natural sunlight, enzyme reduction to DMS
	`S03:S03180`	isotope-labelled tracer addition, antibiotic poisoning, incubation in the dark, filtration
	`S03:S03211`	tracer doping, on-deck incubation
	`S03:S03112`	filtration, incubation in ultra-violet light, enzyme reduction to DMS
	`S03:S0396`	filtration, HgCl2 preservation
	`S03:S03101`	filtration, acidification, combustion
	`S03:S03145`	on-deck light and dark bottle incubation
	`S03:S03451`	acidification, NaOH hydrolysis, NaBH4 reduction
	`S03:S03169`	sediment trapping, volume measurement, drying
	`S03:S0340`	14CO3 doping, incubation in the dark, acidification, scintillant addition
	`S03:S0387`	enzyme digestion, purging, cryogenic trapping
	`S03:S03205`	tracer doping, incubation at unspecified light levels, filtration, acidification
	`S03:S03163`	sediment trapping, acidification
	`S03:S03416`	photolytic conversion to NO
	`S03:S0353`	Millipore prefiltration purging, cryogenic trapping
	`S03:S0318`	on-deck incubation with nutrients
	`S03:S0400`	cascade filtration, acetone extraction
	`S03:S03443`	isotope-labelled tracer addition, incubation, filtration, acidification, gas trapping
	`S03:S0346`	35-S DMSP doping, incubation in the dark, filtration
	`S03:S03402`	drying, grinding, NaOH extraction and filtration
	`S03:S0322`	polymerase chain reaction with True Agg58 primer; serial dilution
	`S03:S0355`	PvI Incubation, cascade filtration
	`S03:S0320`	parallel incubations with and without grazers, acridine orange staining
	`S03:S0401`	isotope-labelled tracer addition, pulse-chase incubations, fixation, SYBR Green I staining, flow sorting
	`S03:S03439`	isotope-labelled tracer addition, on-deck incubation at simulated in-situ light level, filtration, acidification, gas trapping
	`S03:S03120`	filtration, suspension of residue in buffer solution, collection of liberated DMS
	`S03:S03403`	protocol of Barber et al. (1994)
	`S03:S03160`	sediment trapping, acetone extraction
	`S03:S03128`	in-situ incubation dilution experiment
	`S03:S03207`	tracer doping, incubation in the dark
	`S03:S0338`	14C-glucose doping, incubation in the dark, purging with air, acid washing
	`S03:S0335`	variable volume sampling, sieving, picking
	`S03:S0323`	polymerase chain reaction with p1-7 primer; serial dilution
	`S03:S03424`	separation in bromoform
	`S03:S03168`	sediment trapping, solvent extraction
	`S03:S03127`	in-situ incubation
	`S03:S03214`	tracer doping, on-deck incubation at simulated in-situ light level
	`S03:S03144`	on-deck incubation without nutrients, filtration
	`S03:S0367`	antibody doping
	`S03:S03233`	unfiltered seawater incubation in the dark
	`S03:S03151`	persulphate digestion
	`S03:S0336`	0.25M hydroxylamine hydrochloride + 25% acetic acid leaching
	`S03:S03230`	bulk aerosol extraction
	`S03:S03136`	leaching
	`S03:S0402`	filtration, drying
	`S03:S0345`	35-S DMS tracer doping, incubation in the dark, filtration, purging of volatiles
	`S03:S03162`	sediment trapping, acid hydrolysis
	`S03:S03423`	preservation, filtration, SYBR Green I staining, flow sorting
	`S03:S0358`	PvI incubation, filtration
	`S03:S0354`	NaOH hydrolysis
	`S03:S0313`	methanol extraction
	`S03:S03142`	on-deck incubation
	`S03:S0317`	on-deck incubation dilution experiment
	`S03:S03109`	filtration, incubation in natural sunlight, purging, cryogenic trapping
	`S03:S03414`	filtration, water leaching, acid hydrolysis of the leachate
	`S03:S03143`	on-deck incubation with nutrients, filtration
	`S03:S03415`	filtration, water leaching, acid hydrolysis of the residue
	`S03:S0343`	1M sodium acetate leaching at pH 5
	`S03:S0385`	doping with fluorescine diacetate, incubation
	`S03:S0344`	35-S DMS doping, incubation in the dark, filtration
	`S03:S0378`	chloroform extraction
	`S03:S03165`	sediment trapping, methanol extraction
	`S03:S0337`	14C leucine doping, incubation, acidification, purging and trapping CO2
	`S03:S0312`	incubation in filtered seawater
	`S03:S03124`	filtration, hydrolysis
	`S03:S03437`	on-deck incubation at simulated in-situ light level, DCMU poisoning
	`S03:S035`	dilution experiment incubations
	`S03:S03422`	preservation, filtration
	`S03:S03427`	isotopic dilution time-series incubations, filtration
	`S03:S0325`	polymerase chain reaction with universal primer; serial dilution
	`S03:S03140`	not applicable
	`S03:S0316`	not specified
	`S03:S0351`	H2O2 + 1M ammonium acetate leaching at pH 2
	`S03:S0357`	PvI incubation, acidification to remove residual inorganic 14C
	`S03:S03223`	wet sieving, acidification
	`S03:S03234`	parallel incubations with and without added grazers
	`S03:S0348`	Ce-AS reaction
	`S03:S03138`	mesocosm simulated resuspension
	`S03:S03421`	isotope-labelled tracer addition, incubation, filtration, sudan-1 synthesis, SPE extraction, HPLC purification
	`S03:S0342`	1M sodium acetate at pH 5 + 0.25M hydroxylamine hydrochloride leaching
	`S03:S0324`	polymerase chain reaction with p3-42 primer; serial dilution
	`S03:S03190`	tracer doping, incubation at 188uE/m2/s light level, diffusion extraction of gas
	`S03:S03187`	tracer doping, incubation at 100uE/m2/s light level, diffusion extraction of gas
	`S03:S037`	fixed volume sampling, sieving, picking
	`S03:S03146`	on-deck mesocosm incubation

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