Concept
10x Genomics chromium controller single cell analyser
| URI | http://vocab.nerc.ac.uk/collection/L22/current/TOOL2022/1/ | ||
|---|---|---|---|
| Within Vocab | SeaVoX Device Catalogue | ||
| Alternative Labels | 10x chromium controller | ||
| Definition | The Chromium Controller is a single cell gene sequencer and immunity profiler. It uses advanced microfluidics to perform single cell partitioning and barcoding on a timescale of minutes. The instrument utilises gel beads, each containing millions of barcoded oligonucleotides, which are mixed with cells or nuclei from a sample pool at a flow rate designed to enable each gel bead to bind to a single cell or nucleus. Once bound, the gel beads are immersed in an oil solution, capturing each single bead, cell, and the immediately surrounding reagents in a bubble which then serves as a reaction vesicle. Using advanced microfluidics, the instrument encapsulates the gel beads in GEMs (gel beads-in-emulsion), along with a single cell or nucleus and reagents to create a micro reaction. The Chromium Controller enables large scale integrated multiomic analysis, with a cell throughput of 500 to 140,000 singlets per run. The instrument allows for several assays - single cell gene expression, immune profiling, Assay for Transposase-Accessible Chromatin (ATAC), and multiome ATAC + gene expression. | ||
| Date | 2024-03-21T10:48:58 | ||
| Identifier | SDN:L22::TOOL2022 | ||
| Note | accepted | ||
| Is Version Of | TOOL2022 | ||
| version | 1 | ||
| Broader | L05:LAB04 |
autoanalysers | |
| Related | B75:ORG02034 |
10x Genomics | |
L35:MAN0278 |
10x Genomics Inc. | ||
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