Concept
Orange fluorescing microphytoplankton
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200016/2/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | OraMicro | |
| Definition | The Orgmicro group consist of cells > 20 µm They display higher forward scatter (FSC or FWS) and orange fluorescence (FLO) signals than OrgNano and a higher orange fluorescence/red fluorescence ratio compared to the Redmicro group. Staining is not required to distinguish them by flow cytometry. Cells have a high phycoerythrin content and may also contain phycocyanin that can be excited by a red laser. In this case, when cells are excited by a laser beam with a wavelength approximately of 533 nm (green), then they will emit a higher red fluorescence (FLR) signal compared to only chlorophyll a containing cells due to the energy transfer to the pigment phycocyanin that will also emit fluorescence in the FLR channel. This group can include >20 µm cryptophytes, colonial cyanobacterial filaments, but also dinoflagellates and some ciliates. Due to the small volumes usually analyzed by flow cytometry (typically less than 1 cm3), this group is not always properly counted when cells are not abundant enough. When FSC (or FWS) is calibrated by using solutions of beads with different diameters or phytoplankton cell-cultures, it is possible to distinguish microphytoplankton from nanophytoplankton cells with a size close to 20 µm . Chains or colonies may be too large for flow cytometry analysis, depending on instrument characteristics (tubing inner diameter, flow cell dimensions, pulse shape recording or not). | |
| Date | None | |
| Identifier | F0200016 | |
| Note | accepted | |
| Is Version Of | F0200016 | |
| version | 2 | |
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