Concept
Heterotrophic prokaryotes with relatively low nucleic acid
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200011/2/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | Het-LNA | |
| Definition | Heterotrophic prokaryotes include both Bacteria and Archaea. When they do not contain any photosynthetic pigments and thus do not have any autofluorescence property exploitable by flow cytometry, they require staining with a fluorescent dye. In most studies a nucleic acid dye is used. Most of the time, staining of nucleic acids by a green emitting fluorescent dye is used. Blue laser excitation enables heterotrophic prokaryotes to be distinguished in various groups thanks to SSC (or FSC) and FLG signatures : Cells with a lower green fluorescence (FLG) correspond to heterotrophic prokaryotes with a relatively lower nucleic acid content (Het-LNA). Their scatter signals (FSC or FWS, SSC or SWS) are equal or lower than those of Orgpicopro and Redpicoeuk, and may overlap those of Redpicopro. | |
| Date | None | |
| Identifier | F0200011 | |
| Note | accepted | |
| Is Version Of | F0200011 | |
| version | 2 | |
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