Concept
Heterotrophic prokaryotes
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200009/1/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | ||
| Definition | Heterotrophic prokaryotes include both bacteria and Archea. They do not contain any photosynthetic pigments and thus do not have any autofluorescence properties exploitable by flow cytometry. Thus, they require a staining with some fluorescent dye to be resolved by flow cytometry. In most studies a nucleic acid dye is used. Staining of nucleic acids by a dye emitting in the green when excited by a blue laser enables heterotrophic prokaryotes to be distinguished in various groups thanks to sideward scatter (or FWS) and FLG signatures : Cells with a lower FLG correspond to heterotrophic prokaryotes with a Lower Nucleic Acid content (LNA) and cells with a higher FLG correspond to a Higher Nucleic Acid content (HNA). Their scatter signals (FWS, SWS) are lower than those of Synechoccocus and eukaryotic picophytoplankton, and may overlap those of Prochlorococcus. | |
| Date | None | |
| Identifier | F0200009 | |
| Note | accepted | |
| Is Version Of | F0200009 | |
| version | 1 | |
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