Concept
Red only fluorescing microphytoplankton
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200008/4/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | RedMicro | |
| Definition | The Redmico group is a polyphyletic group defined by cells > 20 µm in size. Staining is not required to distinguish microphytoplankton by flow cytometry due to the presence of fluorescent photosynthetic pigments. When excited by a blue laser, this group is discriminated thanks to its forward scatter (FSC or FWS) and red fluorescence (FLR) signals, that are the largest observed by flow cytometry in the microbial community. Due to the small volumes usually analyzed by flow cytometry (less than 1 cm3 in most cases), this group is not always properly counted when cells are not abundant enough. When FSC (or FWS) is calibrated by using beads or phytoplankton cultures, it enables microphytoplankton to be distinguished from nanophytoplankton with a size of about 20 µm. Chains or colony forming cells may be too large for flow cytometry analysis depending on instrument characteristics (tubing size, flow cell dimension, pulse shape recording or not). | |
| Date | 2022-11-13T22:13: | |
| Identifier | SDN:F02::F0200008 | |
| Note | accepted | |
| Is Version Of | F0200008 | |
| Previous Version | 1 , 2 , 3 | |
| version | 4 | |
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