Concept
Red only fluorescing picophytoplankton
URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200004/3/ | |
---|---|---|
Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
Alternative Labels | RedPico | |
Definition | The Redpicoeuk is a polyphyletic group. Based on the literature, this group is defined by eukaryotic phytoplankton cells with a diameter <3 µm (Buitenhuis et al. and references there in, 2012). Staining is not required to distinguish them by flow cytometry due to the presence of fluorescent photosynthetic pigments. The smallest nowadays eukaryotic picophytoplankton known belongs to the genus Ostreococcus. Red only fluorescing eukaryotic picophytoplankton exhibits a well-defined flow cytometry signature when excited with a blue laser, with forward scatter (FSC or FWS) and red fluorescence (FLR) signals larger than those of Redpicopro, but smaller than those of the Rednano. The FSC (or FWS) signal of <3 µm -beads is widely used by the community as an optical standard to localize this group although beads are not a reliable indicator of cell size as measured by flow cytometry. Successive size filtrations or cultures can be used to estimate scatter and size relationship. | |
Date | None | |
Identifier | F0200004 | |
Note | accepted | |
Is Version Of | F0200004 | |
version | 3 |
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