Concept
Red only fluorescing prokaryote picophytoplankton
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200002/3/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | Redpicopro | |
| Definition | The Redpicopro are commonly identified as the unicellular photosynthetic cyanobacteria Prochlorococcus, and are also the smallest marine photosynthetic microorganisms known today. Due to their photosynthetic pigments, staining is not required to detect them by flow cytometry. When excited by a blue laser, their forward scatter (FSC or FWS) and red auto-fluorescence (FLR) induced by chlorophyll signatures (divinyl chlorophyll a for the Prochlorophytes) are the smallest recorded up to now photosynthetic marine microorganisms (Chisholm et al., 1988) and require sensitive photodetectors or high powered lasers, optimally at 445 nm wavelength. They do not emit orange fluorescence because they lack the pigment phycoerythrin. However, at the sea surface, their red fluorescence may be too dim to be detected by flow cytometry, and as a consequence they are often mixed with the background signal (mix between instrumental noise and non-fluorescing particles such as heterotrophic prokaryotes). When well defined (deep water communities, for instance), Redpicopro do not overlap with the background signal. In deep layers, Redpicopro often show a bimodal distribution of the red fluorescence, due to the coexistence of two different ecotypes (Campbell and Vaulot, 1993).In samples incubated with a DNA-staining fluorescent dye and excited with a blue laser to perform heterotrophic prokaryote analyses, Prochlorococcus group(s) can be readily distinguished using sideward scatter(SSC or SWS) vs red fluorescence cytogram, or red fluorescence vs SYBR Green (nucleic acid dye) green fluorescence cytogram. | |
| Date | None | |
| Identifier | F0200002 | |
| Note | accepted | |
| Is Version Of | F0200002 | |
| version | 3 | |
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