Concept
Red only fluorescing prokaryote picophytoplankton
| URI | http://vocab.nerc.ac.uk/collection/F02/current/F0200002/ | |
|---|---|---|
| Within Vocab | Marine Microbial Flow Cytometry Standardised Group Names | |
| Alternative Labels | RedPicoProk | |
| Definition | The RedPicoProk are commonly identified as the unicellular photosynthetic cyanobacteria Prochlorococcus. Due to their photosynthetic pigments, staining is not required to detect them by flow cytometry. When excited by a blue laser, their forward scatter and red auto-fluorescence emitted by chlorophylls (divinyl chlorophyll a for the Prochlorophytes) allow their discrimination from the background noise. These are the smallest marine photosynthetic microorganisms known to date (Chisholm et al., 1988). Their detection requires sensitive photodetectors or high powered lasers, optimally at 445 nm wavelength. They do not emit orange fluorescence because they lack the pigment phycoerythrin. However, at the sea surface, their red fluorescence may be too dim to be detected by flow cytometry and, as a consequence, they are often mixed with the background signal (mix between instrumental noise and non-fluorescing particles such as heterotrophic prokaryotes). When well defined (deep water communities, for instance), RedPicoProk do not overlap with the background signal. In deep layers, RedPicoProk often show a bimodal distribution of the red fluorescence, due to the coexistence of two different ecotypes (Campbell and Vaulot, 1993).In samples incubated with a DNA-staining fluorescent dye and excited with a blue laser to perform heterotrophic prokaryote analyses, Prochlorococcus group(s) can be readily distinguished using sideward scatter vs red fluorescence cytogram, or red fluorescence vs SYBR Green (nucleic acid dye) green fluorescence cytogram. | |
| Date | 2022-11-13T22:13: | |
| Identifier | SDN:F02::F0200002 | |
| Note | accepted | |
| Has Current Version | 5 | |
| Version | 1 , 2 , 3 , 4 | |
| version | 5 | |
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