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Concept

BD Biosciences FACSCalibur Flow Cytometry System

URI http://vocab.nerc.ac.uk/collection/L22/current/TOOL1210/1/
Within Vocab SeaVoX Device Catalogue
Alternative Labels BD FACSCalibur Flow Cytometer
Definition The FACSCalibur flow cytometer is an autonomous benchtop flow cytometer designed for routine cell analysis, assay development, verification and identification of cellular populations. It is equipped with a blue (488 nm) air-cooled argon laser and a red (635 nm) diode laser. For each particle (cell), five optical parameters can be recorded from the 488 nm laser beam excitation: two light scatter signals, namely forward and right angle, and three fluorescences corresponding to emissions in green (530/30 nm BP), orange (585/42 nm BP) and red (670 nm LP) wavelength ranges. A far red fluorescence (661/16 nm BP) induced by the red diode can also be recorded. Data are analysed using BD Biosciences CellQuest software. Optional features include a cell sorting option, allowing users to identify and isolate a population of interest and a HTS option (High-throughput (HT) or Standard (STD) mode), where sample volumes range from 2-10 microlitres in HT mode and 2-200 microlitres in STD mode. An optional BD FACS Loader tube-lifter can be used to verify tube position and rack identification. The instrument has a capture rate of 300 cells per second, supports 40 (12 x 75 mm) tubes per rack, and has an operating temperature ranging from 16-29 degC.
Date 2018-02-02T12:39:33
Identifier SDN:L22::TOOL1210
Note accepted
Is Version Of TOOL1210
version 1
Broader L05:LAB37 flow cytometers
Related B75:ORG00623 BD Biosciences

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