@prefix cpm: . @prefix dc: . @prefix dcat: . @prefix dce: . @prefix dct: . @prefix grg: . @prefix iop: . @prefix ns0: . @prefix ns1: . @prefix owl: . @prefix pav: . @prefix prov: . @prefix puv: . @prefix qudt: . @prefix rdf: . @prefix rdfs: . @prefix reg: . @prefix semapv: . @prefix skos: . @prefix sssom: . @prefix void: . rdf:type skos:Concept ; dce:identifier "SDN:L22::TOOL1782" ; dc:date "2022-03-22 14:49:18.0" ; dc:identifier "SDN:L22::TOOL1782" ; pav:authoredOn "2022-03-22 14:49:18.0" ; pav:hasCurrentVersion ; pav:version "1" ; void:inDataset ; owl:deprecated "false" ; owl:versionInfo "1" ; skos:altLabel "BD FACScan" , "BD FACScan"@en ; skos:broader ; skos:definition "A flow cell-based benchtop cytometer. It uses hydrodynamic focusing to pass particles through a laser light. The scattered and fluorescent light is collected by appropriately positioned lenses. A combination of beam splitters and filters steers the light to the appropriate detectors. The detectors produce electronic signals proportional to the optical signals striking them. The analyser uses a 20mW Coherent 488nm, air cooled, argon ion laser. Detectors are three photomultiplier tubes with bandpass filters for FITC (530/30nm), PE (575/26nm), PerCP, or PE-Cy5,or PE-Cy5-5 or PE-Cy7 (650nmLP). Fluorescence sensitivity: <2,000 molecules of equivalent soluble fluoroscein. Fluorescence resolution: < 3% for PI labelled chick erythrocyte nuclei."@en ; skos:inScheme ; skos:notation "SDN:L22::TOOL1782" ; skos:note "accepted"@en ; skos:prefLabel "Becton Dickinson FACScan flow cytometer"@en ; skos:related , .